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Pellets were resuspended in 300 l PBS, and 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)

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More importantly, in the studies conducted in Sardinian subjects with G6PD deficiency, the possible copresence of beta thalassemia with a prevalence of up to 12% in the general population [100]was not taken into account

Impaired glucose tolerance in susceptible (pre-diabetic) individuals could result in elevated blood glucose concentrations and clinical type 2 diabetes mellitus

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