In our official GST affinity purification protocol we recommend using 1x PBS, pH 7.3 as the binding buffer
Livraghi-Butrico A, Grubb BR, Wilkinson KJ, Volmer AS, Burns KA, Evans CM, O'Neal WK, Boucher RC
Briefly, mice were housed individually in standard laboratory cages equipped with two bottles, one containing water and the other containing a solution of ethanol
Glycaemic improvements were maintained in Zucker diabetic fatty rats 3 weeks after treatment cessation even though they had gained body weight and were similar in mass to vehicle-treated controls, demonstrating that the tri-agonist delays T2D progression in rodent models of spontaneous diabetes
For dietary green tea (brewed, with meals), timing relative to isotretinoin is less critical given the far lower EGCG concentrations involved