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For purification, a cell pellet was resuspended in lysis buffer containing 50 mM Tris pH 7.5, 500 mM NaCl, 50 ug/ml DNase1, 50 ug/m lysozyme, 0.5 mM Benzamidine, 0.5 mM PMSF, and EDTA Free protease inhibitor cocktail (Roche), sonicated and debris removed by a 45-min centrifugation at 50,000 x g

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