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The cells were washed in PBS containing 0.5% BSA and centrifuged at 1500 g for 4 min twice, after which the cells were fixed in IC fixation buffer (cat#FB001C, BD Biosciences Pharmingen, CA, US) at 4 C for 10 min

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Exenatide treatment led to the augmented activation of proteins involved in insulin signaling in the brain and of effectors, such as Akt and mTOR

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Instead, she carefully monitors each persons reaction to the medication, tailoring the approach based on their bodys unique response