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Cell Counting kit (CCK-8) viability and cell death assay Cells were seeded into 96-well plates at a density of 3 10 cells per well and subjected to the designated treatments for 72 h

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Long-term considerations include the recognition that tirzepatide is a chronic disease management medication rather than a short-term intervention

nih An acute exercise bout can trigger MOTS-c to translocate into nuclear regions of skeletal muscle, although more work is needed to validate this response