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Following SC administration into the fatty area of the abdomen, the subcutis (the layer of skin directly below the dermis and epidermis, collectively referred to as the cutis), apomorphine is rapidly absorbed and the T max ranges from 10 to 40 min

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Then cells were resuspended in tag-mentation buffer (10 mM MgCl2 in Dig-med Buffer) and incubated at 37 C for 1 h